RNA extraction

RNA extraction is the purification of RNA from biological samples. This procedure is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA.[1] Several methods are used in molecular biology to isolate RNA from samples, the most common of these is Guanidinium thiocyanate-phenol-chloroform extraction.[2][3]

See also

References

  1. ^ Peirson SN, Butler JN (2007). "RNA extraction from mammalian tissues". Methods Mol. Biol.. Methods in Molecular Biology 362: 315–27. doi:10.1007/978-1-59745-257-1_22. ISBN 978-1-58829-417-3. PMID 17417019. 
  2. ^ Chomczynski P, Sacchi N (2006). "The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on". Nat Protoc 1 (2): 581–5. doi:10.1038/nprot.2006.83. PMID 17406285. 
  3. ^ Bird IM (2005). "Extraction of RNA from cells and tissue". Methods Mol. Med. 108: 139–48. PMID 16028681. 
Overview
Element
Linked Life
Engineering
Concept
Technique

Cell culture • model organisms (such as C57BL/6 mice) • method (Nucleic acid • Protein) • Fluorescence, Pigment & Radioactivity

High-throughput Technique (-omics): DNA microarray • Mass spectrometry • Lab-on-a-chip